|
KMID : 1098420050130050219
|
|
Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 5 p.219 ~ p.226
|
|
|
Protective Effect of Sanguisorba officinalis L. Root on Amyloid {beta} Protein (25-35)-induced Neuronal Cell Damage in Cultured Rat Cortical Neuron
|
|
Ban Ju-Yeon
Cho Soon-Ock
Jeon So-Young
Song Kyung-Sik
Bae Ki-Hwan
Seong Yeon-Hee
|
|
|
Abstract
|
|
|
|
Sanguisorbae radix (SR) from Sanguisorba officinalis L. (Losaceae) is widely used in Korea and China due to its various pharmacological activity. The present study aims to investigate the effect of the methanol extract of SR on amyloid {beta} Protein(25-35) (A{beta};(25-35)), a synthetic 25-35 amyloid peptide, -induced neurotoxicity using cultured rat cortical neurons. SR, over a concentration range of 10-50;{mu}g/ml, inhibited the A{beta} (25-35) (10;{mu}M)-induced neuronal cell death, as assessed by a 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. Pretreatment of SR (50;{mu}g/ml) inhibited 10;{mu}M;A{beta} (25-35)-induced} elevation of cytosolic calcium concentration ([Ca^{2+}]c), which was measured by a fluorescent dye, fluo-4 AM. SR (10;and;50;{mu}g/ml) inhibited glutamate release into medium induced by 10;{mu}M;A{beta}(25-35), which was measured by HPLC, and generation of reactive oxygen species. These results suggest that SR prevents A{beta} (25-35)-induced neuronal cell damage in vitro.
|
|
|
KEYWORD
|
|
|
Sanguisorbae radix, Neuroprotection, amyloid {beta} Protein(25-35), Neurotoxicity, Cortical neurons
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|